摘要
本试验筛选到一株适于使苹果汁澄清的果胶酶产生黑曲菌No.07,应用物理因素及化学因素对No.07进行处理,从白色突变菌落中获得一高产株CL—8501,对CL—8501的培养基及发酵条件进行了优化,在优化的培养基及较适条件下,CL—8501产酶达631.6单位/毫升,产量此出发株No.07提高约105倍。
Aspergillus niger No.07, Which is suitable for clarification of apple juice, is screened, then treated with physical and chemical factors. A high yield strain CL-8501 is selected from white mutant colonies. The fermentation conditions and medium composition of CL-8501 are investigated. Under the favorable condition and in the suitable medium(sucrose2%, pectin 2%, NH_4NO_3 0.2%), the activity of pectinase of CL-8501 reaches 631 6U/ml, 105 times higher than the starting strain No. 07.
关键词
果胶酶
黑曲菌
菌种选育
Pectinase
Aspergillus niger
Apple juice clarification
Strain selection
