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转基因烟草定性检测方法的研究 被引量:8

Study on the qualitative determination of genetically modified tobacco
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摘要 本文总结多年的试验研究结果,提出了一套较为完善的转基因烟草定性检测技术,应用该技术,能使转基因烟草的检测精确度由常规PCR的0 . 1%提高到0 . 0 0 5 %。该技术在两次CORESTA转基因烟草盲检合作试验及多年的进出口烟叶、卷烟制品的转基因检测工作中得到广泛的验证。 The technique of qualitative determination of GM tobacco was established after several years of researche. With the methods, the accuracy of GM tobacco determination was increased to 0.005% compared with 0.1% of normal PCR. This technique has been verified by two rounds of CORESTA GM tobacco ring trials and has been used in GM tobacco determination works.
出处 《中国烟草学报》 EI CAS CSCD 2005年第2期24-27,共4页 Acta Tabacaria Sinica
基金 国家烟草专卖局科研项目资助 (合同号 1 1 0 2 0 0 1 0 1 0 0 8)
关键词 巢式PCR 烟草及制品 定性转基因检测 Nest-PCR Tobacco GM quantitative detection
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