摘要
目的探讨FasL基因转移后造血细胞活性是否受到影响,骨髓移植(bonemarrow transplantation,BMT)后受者造血重建是否受影响。方法常规收集Balb/C小鼠和BAC小鼠股骨和胫骨骨髓单个核细胞(bonemarrowmonomuclearcells,BMMNC),采用脂质体转移法将FasL基因转入Balb/C小鼠BMMNC,然后按1∶0.625体外与BAC鼠BMMNC混合培养,6d后进行实验。取8周龄Balb/C小鼠经致死量照射后随机分为4组。第1组10只(空白对照组:未移植细胞);第2组10只(未转染基因Balb/C鼠BMMNC+BAC鼠BMMNC);第3组10只(转基因Balb/C鼠BMMNC+BAC鼠BMMNC);第4组10只(同基因BMT组)。观察移植后受者鼠造血重建及细胞来源,移植物抗宿主病(graftversushostdisease,GVHD)及生存率。结果BMT+10d(移植后为“+”),+20d,第4组白细胞及血小板计数明显高于第2、3组(P<0.01);第2、3组间差异无统计学意义(P>0.05);+30d,第2、3、4组间差异无统计学意义(P>0.05);第1组小鼠相继于1周内死亡,死亡后取脾观察未见脾结节形成。第2、3组存活的11只雌性Balb/C小鼠BMMNC中,Y染色体分析表明,各组均有供者来源的造血干细胞植入。第2、3、4组2个月生存率分别为30%、80%、100%。第3组生存率明显高于第2组(P<0.01),与第4组比较差异无统计学意义(P>0.05)。第3组中2只及第2组中9只鼠出?
Objective The study was designed to explore the influence of transfected bone marrow mononuclear cells (BMMNC) transplantation on the hematopoietic cells activity and the recipient mice hematopoietic reconstitution. Methods The exogenous mFasL-cDNA gene was transferred to Balb/C mouse BMMNC by liposomes. Then the transferred BMMNC was co-cultured with BMMNC from BAC mouse (H-2d×b, male) at a ratio of 0.625 to 1 for 6 days. In the experimental group (the 3rd group), 1×10 7 (0.5 ml) mixed viable cells were injected into whole bodily irradiated ( 60Co-r) mice (6 to 8 week old female Balb/C) via the tail vein. The following grafted mice were simultaneously used in the study, the mice transplanted with 0.5 ml of culture medium, the mice transplanted with the mixture of untransferred Balb/C mouse BMMNC and BAC mouse BMMNC, the mice transplanted with the mixture of transferred Balb/C mouse BMMNC and BAC mouse BMMNC and the mice transplanted with Balb/C mouse BMMNC. The hematopoietic reconstitution, the origin of bone marrow cells responsible for the reconstitution, the graft versus host disease (GVHD), the survival rate for the recipient mice were observed after bone marrow transplantation (BMT). Results The counts of leukocytes and platelets in recipient blood of group four on +10 d and +20 d after BMT were higher than those in group three and group two (P<0.01), but on +30 d after BMT the counts of leukocytes and platelets in recipient blood of group two, group three and group four were at their normal levels. The Y chromosome from donor mice was discovered in BMMNC of recipient mice having survived for over two months after BMT in group two and group three. The survival rate of the recipient mice two mouths after BMT in all groups were 0% for group one, 30% for group two, 80% for group three, and 100% for group four, respectively. The total survival rate of recipient mice in the experimental group was obviously higher than that of group two (P<0.01). Grade Ⅱ to Ⅲ GVHD signs were found on the histology from dead
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2005年第4期284-287,共4页
Chinese Journal of Pediatrics
基金
国家自然科学基金资助项目(399770767)
