摘要
采用不同培养基对MT09酿酒酵母进行不同条件下的生孢培养实验,通过菌体前培养和诱导生孢子、单倍体细胞的分离、单倍体生孢能力的确认、菌体生长曲线的绘制、单倍体菌株的诱变和营养缺陷型的筛选,得出单倍体菌株D3作为最好的诱变出发菌株。最后筛选出菌株D3-02为遗传稳定性的呼吸缺陷型突变株。(孙悟)
Spore-producing experiments of MT09 (distillers yeast) were done (using different culture mediums and under different conditions). As a result, haploid strain D3 was obtained through thallus culture and inducement, separation of haploid cells, identification of spore-producing capability of haploid, drawing of thallus growing curve, mutation of haploid strain, and breeding of haploid respiration defect type. D3 was used as the best mutation startup strain and finally D3-02 was screened as respiration defect mutant strain of genetic stability. (Tran. by YUE Yang)
出处
《酿酒科技》
北大核心
2005年第5期45-47,共3页
Liquor-Making Science & Technology
关键词
微生物
酿酒酵母
单倍体
呼吸缺陷型
选育
microbe
distillers yeast
haploid
respiration defect type
breeding
