摘要
目的 检测急性胰腺炎早期转化生长因子- β1(TGF - β1)信使RNA的表达,Ⅰ型胶原(Collagen -Ⅰ)以及α-平滑肌激动蛋白(α-SMA)的生成,探讨胰腺星形细胞在胰腺炎早期纤维化启动中的作用及其意义。方法 采用阻断胰腺导管同时腹腔注射雨蛙素(5 0 μg·kg-1·d-1)的方法诱导急性胰腺炎大鼠模型,假手术组施行开腹手术并腹腔注射生理盐水。分别于0、72、12 0h处死大鼠,取完整胰腺组织用免疫组化方法检测Ⅰ型胶原和α-SMA的表达,再以逆转录聚合酶链反应(RT -PCR)检测TGF - β1mRNA的表达情况。结果 假手术组大鼠胰腺组织胰腺星形细胞染色偶尔阳性,实质无明显改变,胶原染色阴性,TGF - β1mRNA无表达。模型72h组胰腺炎症改变,Collagen -Ⅰ、α-SMA检测表现胰腺间质以及腺泡周围的黄染,涉及广泛,深浅不一,较正常组变化明显;TGF - β1mRNA表达明显增强。模型12 0h组,光镜检查炎症表现更甚,间质组织增生。Collagen -Ⅰ、α-SMA表现也呈进行性进展,TGF - β1mRNA表达比较72h模型组略有降低。结论 研究表明,在急性胰腺炎早期即出现TGF - β1的激活,并刺激胰腺星形细胞(α-SMA阳性细胞)激活、增生,并使Collagen -Ⅰ逐渐生成沉积,提示胰腺的纤维化进程的启动,且随时间延长胰腺星形细胞激活逐渐增多。
Objective To study the significance and role of pancreatic stellate cells (PSCs) in pancreatic fibrosis in the early time of acute pancreatitis by determining the expression of transforming growth factor-β_1(TGF-β_1) messenger RNA and deposition of collagen- Ⅰ,α-smooth muscle actin(α-SMA)in acute pancreatitis. Methods Acute pancreatitis was induced in rats by hyperstimulation with cerulein(50 μg·kg -1 ·day -1 ) and concurrent pancreatic duct obstruction for 72 and 120 hours. Sham animals were injected pure water and underwent laparotomy and manipulation of the pancreas without duct obstruction. Entire pancreas was harvested at the timepoint of 0,72 and 120 hours. Immunohistochemistry was used for examining collagen-Ⅰ and α-smooth muscle actin antibodies. Messenger RNA of transforming growth factor-β_1 (TGF-β_1) was detected by reversal transcription-polymerase chain reaction(RT-PCR).Results There were occasional α-SMA-positive cells (pancreatic stellate cells) in the sham animals and there was none for collagen-Ⅰ staining. Within 72 hours in model animals, α-SMA-positive cells appeared around pancreatic acini. The appearance of these cells were followed by collagen-Ⅰpositive staining. At 120 hours, the above staining progressed gradually. TGF-β_1 mRNA was not found in sham animals.At 72 hours and 120 hours, it showed obvious expression,and expression at 72 hours is a little more than that at 120 hours. Conclusions TGF-β_1 stimulated by acute pancreatic inflammation results in activation of pancreatic stellate cells (α-SMA-positive cells ) and stellate cells gradually secrete collagen and matrix which leads to pancreatic fibrosis finally.
出处
《中国急救医学》
CAS
CSCD
北大核心
2005年第5期341-343,共3页
Chinese Journal of Critical Care Medicine
基金
全军医学科研"十五"计划重点课题 (No 0 1Z0 74)
