摘要
背景:纳洛酮对各种类型的昏迷具有显著的促醒作用。一般认为纳洛酮的促醒作用与抑制内源性阿片肽(主要为β内啡肽)有关,但昏迷的程度并非一定与β内啡肽水平呈正相关。目的:在已证实促醒剂纳洛酮对皮质神经元具有直接兴奋作用的基础上,进一步研究阐明纳洛酮的兴奋皮质作用是否具有剂量依赖性。设计:以细胞为研究对象的单因素设计。单位:一所大学医院的神经内科,一所军医大学医院的神经内科。材料:实验在华中科技大学同济医学院实验中心完成。选取出生8～12d健康Wistar大鼠30只,体质量150～250g,雌雄不限。方法:实验在20～24℃的室温下进行。灌流槽置于倒置显微镜载物台上,选择表面光洁,胞体呈三角型或锥形且折光性强,有一个以上突起的神经元进行膜片钳实验。以急性分离Wistar大鼠额叶皮质锥体细胞为研究对象,采用膜片钳全细胞记录技术,观察不同剂量纳洛酮作用下,皮质神经元膜电位和自发性电活动频率变化的差异。主要观察指标:不同剂量纳洛酮的兴奋反应率、去极化幅度和自发电活动增加的比率。结果:急性分离的皮质神经元对100,50,10,1,0.1μmol/L等纳洛酮的兴奋反应率分别为83%,67%,86%,71%,33%,对应的去极化幅度分别为9.8,9.6,8.4,5.2和1.3mV,自发电活动增加的比率分别为587%,375%,291%,125%,69%。结论:
BACKGROUND:Naloxone has a significant arousal effect on many types of comas.It is usually believed that this is because its inhibition on endogenous opioid peptides.But depth of coma is not necessarily positively correlated to endorphin(EP). OBJECTIVE:Based on existing findings on direct stimulating effect of naloxone on cerebral cortex,further studies need to be done to explore whether it is dose dependent or not. DESIGN:Single factor design based on cells. SETTING:Neurology department in a university hospital and the neurology department in a hospital of a military medical university of Chinese PLA. MATERIALS:This study was completed in the Laboratory Center of Tongji Medical College,Huazhong University of Science and Technology.Thirty healthy new born Wistar rats,regardless of their gender,aging 8-12 days and weighing 150-250 g,were selected. METHODS:The experiment was performed at room temperature.The perfusion slot were placed on the microscope stage,and cells with smooth surfaces,triangle or pyramidal shapes,strong refraction and more than one neurites were selected for patch clamp experiment. Patch clamp whole cell recording technique was used to measure the pyramidal cells of the frontal lobe immediately after separated from the Wistar rats,and to investigate the fluctuations of their membrane potential of cerebral cortex neurons and the frequencies of their spontaneous electric activities after administration of naloxone at different doses. MAIN OUTCOME MEASURES:The neural excitatory reaction rate,depolarization amplitude and increasing rate of spontaneous electric activities after administration of different doses of naloxone were selected as main outcome measurements. RESULTS:The excitatory reaction rates of cerebral cortex neurons immediately after separation to doses of naloxone(100,50, 10,1,0.1 μmol/L) were 83
出处
《中国临床康复》
CSCD
北大核心
2005年第13期204-205,共2页
Chinese Journal of Clinical Rehabilitation
