摘要
目的研究SurvivinmRNA在膀胱移行细胞癌(TCC)患者尿脱落细胞中的表达,及其与TCC的发生发展、病理分级、临床分期的相关性。方法用半定量逆转录-聚合酶链反应(RT-PCR)法对30例TCC患者、18例健康志愿者的新鲜中段晨尿Survivin的mRNA进行检测。结果病例组和正常对照组尿脱落细胞中SurvivinmRNA的阳性率分别为100.00%和22.22%。病例组和对照组SurvivinmRNA表达阳性率差异有统计学意义(P<0.01)。在病例组中,按TCC病理分级(I级、Ⅱ级、Ⅲ级)分组的标本灰度比值分别为1.00±0.18,1.28±0.14,1.70±0.23,各组间差异有统计学意义(P<0.01)。按TCC的临床分期(未突破基底膜、侵及浅肌层、侵及深肌层)分组的标本灰度比值分别为1.01±0.17,1.26±0.14,1.70±0.23,各组间差异有统计学意义(P<0.01)。结论检测尿脱落细胞中SurvivinmRNA的表达可用于TCC患者的诊断及恶性程度和预后的判断。
Objective To explore the expression of Survivin mRNA in exfoliate cells in urine of the patients with transitional cell carcinoma of bladder (TCC),and its relation with the occurrence,development,differentiation and pathologic grading and clinical staging of TCC.Methods Fresh urine of 30 patients with TCC and 18 healthy volunteers who voided the second urine in morning was collected.The expression of Survivin mRNA in the urine was detected by semiquantitative reverse transcription polymerase chain reaction (RT-PCR).Results The positive rate of the Survivin mRNA expression in the exfoliate cells in urine was 100% in the patient group and 22.2% in the control group respectively.There were significance between them.The gray degree ratios of the Survivin mRNA to the β-actin mRNA varied accordingly to different pathologic grades and clinical stages (grade I: 1.00± 0.18, grade Ⅱ: 1.28± 0.14, grade Ⅲ: 1.70± 0.23, without break through basement membrane: 1.01± 0.17, superficial muscle invasion: 1.26± 0.14, deep muscle invasion: 1.70± 0.23), and there was significant difference between them (P< 0.01). Conclusion Detecting Survivin mRNA in the urine can be used in diagnosing TCC,judging the malignancy degree of TCC and the prognosis of TCC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第5期570-571,共2页
Chinese Journal of Experimental Surgery
基金
江苏省重点人才基金资助项目(RC2003094)
江苏省卫生厅科技基金资助项目(H200146)
