摘要
用杂交瘤技术建立抗KDRFc的杂交瘤细胞系,以KDRFc抗原免疫雌性BALB/c小鼠,取其脾细胞与SP2/0骨髓瘤细胞融合,经ELISA间接法检测和有限稀释法克隆培养,再经稳定性检测、效价检测,筛选出分泌抗KDRFc单克隆抗体的杂交瘤细胞.得到了10株能持续分泌抗KDRFc的单克隆抗体的细胞株,其中3株分泌的单抗具有较高的生物活性、特异性和稳定性.
In order to establish anti-KDR-Fc antibody producing hybridoma cell lines,female BALB/c mice are immunized with the purified antigen of KDR-Fc, and their spleen cells are fused with SP2/0 cells to raise hybridomas. After inoculation for several times, the positive of clones are screened by indirect ELISA,and the characteristics of the antibodies are detected. Ten hybridoma cell lines are established,three of them, 1D4,4G3 and 9F10 has high biological activity,specificity and stability.
出处
《西北师范大学学报（自然科学版）》
CAS
2005年第3期71-74,共4页
Journal of Northwest Normal University(Natural Science)
