摘要
通过ＡＰＡＡＰ桥联酶标技术检测接触不同浓度甲醛人群外周血淋巴细胞亚群（Ｂ细胞、ＣＤ３、ＣＤ４、ＣＤ８）。对照组、学生组和工人组、服务员组接触甲醛的浓度分别为：０．０１１ｍｇ／ｍ３、０．５０８ｍｇ／ｍ３、０．７４ｍｇ／ｍ３、０．１０７ｍｇ／ｍ３。结果发现；学生与工人组中除工人组的ＣＤ４外的其它亚群与对照组相比其差异均有显著性（Ｐ＜０．０５）．Ｂ细胞和ＣＤ４／ＣＤ８比值升高．ＣＤ３、ＣＤ４、ＣＤ８下降；男性ＣＤ３下降明显高于女性．服务员组和对照组的各亚群差异无显著性（Ｐ＞０．０５）。提示：甲醛可以对人免疫细胞产生多种毒性，短期接触者和男性尤为明显。
The peripheral lymphocyte subsets (B cell. CD3、 CD4、CD8 ) of populations exposed to different concentrations of Formaldehyde(FA)were measured using APAAP bridged enzyme mark technique. FA concentrations of control group.students group、FA-producing workers.servers in ballroom were respectively 0. 011mg/m3. 0. 508mg/m3. 0. 747mg/m3. 0.107mg/m3. Subsets of students .workers except CD4 of workers had statistically significant difference compared with control group (p<0. 05),the percentages of CD3 、CD4 and CD8decreased, the percentage of B cell and value of CD4/CD8rose.The decline of the percentage of CD3 of male was more obvious than famale S. There were no difference between subsets of control group and servers (P> 0. 05). It was suggested that FA could result in various immune cell toxicity,esgecially in male and short time exposure.
关键词
甲醛
淋巴细胞
毒性
Human lymphocyte subsets Formaldehyde (FA)APAAP method
