摘要
目的 通过体外实验研究E2、MPA、HB -EGF对Ishikawa细胞中MMP - 9/TIMP - 1mRNA表达的调节,探讨其在胚胎种植中的意义。方法 体外培养Ishikawa细胞,分别加入E2、MPA、E2 +MPA、E2 +MPA +RU4 86、HB -EGF刺激4 8h后,采用原位杂交、RT -PCR测定各种条件下MMP - 9、TIMP - 1mRNA的表达。结果 雌、孕激素单独或联合作用均可以显著降低TIMP - 1mRNA的表达(P <0 .0 5 ) ,同时加RU4 86后TIMP - 1mRNA的下降趋势减弱。相反HB -EGF使TIMP - 1mRNA的表达增高(P <0 .0 5 )。Ishikawa细胞中MMP - 9mRNA均没有阳性表达。结论 (1)雌、孕激素对TIMP - 1mRNA具有下调作用,RU4 86可以抑制孕激素的作用。(2 )HB -EGF对TIMP - 1mRNA的表达具有上调作用。(3)MMP - 9mRNA在Ishikawa细胞中没有表达。
Objective: To investigate the regulatory effect of multifactor (E2, MPA, HB-EGF) on MMP-9 mRNA and TIMP-1 mRNA in Ishikawa cells. Methods: Ishikawa cells were incubated with E2 (10 -8 M), MPA(10 -6 M), RU486 (10 -8 M) or HB-EGF (10ng/ml) for 48 hours respectively. The expression of MMP-9 mRNA and TIMP-1 mRNA were detected by in situ hybridization, reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Either estrogen alone, progestin alone or estrogen combined with progestin could significantly decrease the expression of TIMP-1 mRNA ( P <0.05). But RU486 could inhibitor the effect of down-regulation. On the contrary, HB-EGF elevated the expression level of TIMP-1 mRNA ( P <0.05). The expression of MMP -9 mRNA does not be detected in Ishikawa cells. Conclusions: (1) Both estrogen and progestin can down-regulate the expression of TIMP-1 mRNA in Ishikawa cells, but RU486 can inhibitor the effect. (2) HB-EGF can elevate the level of TIMP-1 mRNA.(3)There is no expression of MMP-9 mRNA in Ishikawa cells.
出处
《中国优生与遗传杂志》
2005年第4期13-15,共3页
Chinese Journal of Birth Health & Heredity
