摘要
目的:建立高效液相-荧光检测法测定血浆替米沙坦浓度。方法:采用Symmetry C18柱,流动相:0.05 mol·L-1磷酸二氢钾溶液(用1 mol·L-1磷酸溶液调节pH=3.65):乙腈=55:45,激发波长:300 nm;发射波长:385 nm。结果:线性回归方程Y =0.055 80C-0.001 45,r=0.999 8;线性范围为1.0～500.0μg·L-1,最低检测限为1 μg·L-1。低、中、高三种浓度的绝对回收率分别为77.6%,94.97%和97.66%,相对回收率为96.62%,98.46%和104.43%,日内,日间RSD均小于10%。结论:该法操作简便,精密度高,可满足生物利用度试验方法学的要求。
Objective: To establish a HPLC for the determination of concentration of telmisartan in human plasma. Method: The samples were chromatographed on a symmetry-C18 reversed-phase column and monitered by fluorescene-detection at the EX300 and EM385 nm. The mobile phase consisted of 0. 05 mol·L-1 of potassium dihydrogen phosphate-acetonitrile(55:45,pH=3. 65) with a flow rate of 1. 0 ml·min-1. Result: The calibration curve was linear within a range of 1.0-500 μg·L-1 ( r = 0. 999 8). The RSD of in-tra-day and inter-day were less than 10% . The absolute recovery of three kinds of concentration were 96. 6% ,98. 46% , and 104. 43% , respectively. The limit of quantificaiton was 1 μg·L-1. Conclusion: This method had a good selectivity,sensitivity, and reproducibity. This method was shown to be suitable for pharmacokinetic study on telmisartan in human, and monitoring the plasma level of drug in clinic.
出处
《中国药师》
CAS
2005年第5期405-407,共3页
China Pharmacist
