摘要
从人低分化鼻咽癌细胞系（ＣＮＥ－２Ｚ）中分离出单克隆株４－２－Ｈ５、５－１－Ｆ＿１、５－２－Ｆ＿７进行体外培养，分别计算各克隆株细胞倍增时间和分裂指数，用玻璃微电极细胞内记录方法测量细胞膜电位。结果表明：（１）随培养时间延长，三个克隆株细胞膜电位负值都减小，差异有显著意义；（２）膜电位负值下降率与细胞株生长增殖能力呈正相关，（３）Ｈ＿５，Ｆ＿１细胞膜电位负值与细胞外Ｋ￣＋浓度的对数值接近反比关系。提高培养液Ｎａ￣＋浓度。ｈ＿５、ｆ＿１细胞膜电位负值稍下降，前者无显著性意义，后者有显著性意义，提示Ｈ＿５、Ｆ＿１细胞膜电位主要与Ｋ￣＋外流有关，在Ｆ＿１细胞，膜电位形成还与Ｎａ￣＋有一定关系。
Three clonal strains(4-2-H_5,5-1-F_1 5-2-F_7)were derived from the humanepithelial cells of poorly differentiated nasopllaryngeal carcinoma(CNE-2Z )and culturedin vitro. The population-doubling time and the mitotic indexes of these clones were calcu-lated. The membrane potentials of these cells were recorded intracellularly using glass mi-croelectrodes. The results indicated:(1) The negative value of the membrane potentials de-creased significantly while the culture time was prolonged;2)The decreased rate of negativemembrane potential correlated positively to the growth ability of cells;(3 ) The value ofthe negative membrane potentials of H_5 and F_1 cells was inversely proportional to lg( K￣+)o.The negative membrane potentials of F_1 cells decrcased slightly( P <0.05 ) and those of H_5cells did not change very much while(Na￣+)o was increased. These results suggested that thetormation of the membrane potential was mainly related to outward diffusion of( K￣+)_i inH_5 and F_1 cells, and slightly related to inward diffusion of(Na￣+)o in F_1 cells.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1994年第1期68-72,共5页
Chinese Journal of Pathophysiology
关键词
鼻咽肿瘤
克隆细胞
膜电位
Nasopharyngeal neoplasms
Clone cells
cells cultured
Membrane potentials
