摘要
应用５００、１５００单位人体注射用的盐酸四环素和青霉素Ｇ钾浸渍处理龙眼鬼帚病苗，均无抑制病状表现，表明本病不是由ＭＬＯ和ＢＬＯ引起的；电镜观察仅在病叶组织筛管细胞内见到成束分布在细胞质内的线状病毒粒体；通过Ａ蛋白。免疫电镜检测，亦可在龙眼鬼帚病介体昆虫荔枝蝽（ＴｅｓｓａｒａｔｏｍａｐａｐｉｌｌｏｓａＤｒｕｒｙ）、龙眼角颊木虱（ＣａｒｎｅｇｅｎａｐｓｙｌｌａｓｉｎｉｃａＹａｎｇｅｔＬｉ）唾液腺内捕获到与病叶组织相同的病毒粒体，从而进一步肯定本病病原的病毒性质。
he results of longan seedlings with witches'broom disease immersed in tlie solutions of tetracycline-HCl(500,1500ppm for four hrs)and penicillin-GK(5000 ppm for four hrs)showed that thesymptom of the diseased seedlings were unable to be inhibited,which indicated that the disease was notcaused by MLO and BLO.The bunchy filan1entous viruses were found in the sieve cells of the diseasedleaf under electron micrcacope,no virus particle were deteeted in healthy plant used as control.Thevirus particle simil lar to that existed in the diseased leaves can be trapped from the salivary glands of Tessaratoma papillosa Drury(adults)and Cornegenapsylla sinica Yang et Li(adults)by SPA-ISEMmethod.It is concluded that the virus particles are the causal agent of Iongan wi tches'broom disease.
出处
《中国病毒学》
CSCD
1994年第2期138-142,共5页
Virologica Sinica
基金
福建省科委重点研究课题
关键词
龙眼
鬼帚病
病毒
Longan,Witches'broom disease,Virus
