摘要
目的构建果实特异性启动子驱动的含编码变形链球菌唾液粘附区(sbr)植物表达载体,进一步提高外源目的基因的表达,为研制有效的转基因植物防龋疫苗打下基础。方法提取番茄基因DNA,利用PCR技术扩增果实特异性启动子E8和2A11基因,双酶切质粒PROP及PROSC,分别与目的基因连接,构建重组植物表达载体。结果通过双酶切鉴定,目的基因片段已正确整合到植物表达载体中。结论本实验成功构建了果实特异性启动子驱动的含sbr基因的植物表达载体。
Objective To construct a plant effective expre ssion vector driving by a fruit specific promoter for the expression of Strptomyce mutant sbr;to further improve the expression of exogenous gene in plant,and to p repare for the development of effective anti-caries vaccine.Methods Genomic DNA was extracted from tomato,and the fruit specific promoters of gene E8and 2A11were amp lified using PCR technique and cloned into plasmid PROP and PROSC respectively.The recombinant expr ession vector containing the fruit s pecific promoter and target gene was constructed.Result The target gene was successfully int egrated into the plant expression ve ctor and confirmed by dual-enzyme digestion.Conclusion In this study plant expression vecto r containing sbr gene driven by fruit specific promoter was successfully constructed.
出处
《热带医学杂志》
CAS
2005年第2期135-138,共4页
Journal of Tropical Medicine
基金
国家自然科学基金(No.30240054)
广东省自然科学基金(No.001364)
广东省科技计划项目基金(No.2KM05402S)。
