摘要
利用地高辛DNA标记与检测技术反向Northern斑点杂交,对小尾寒羊和滩羊卵巢组织差异显示ESTs进行鉴定,避免同位素放射性污染,安全性很高且操作简单,提高了实验的准确性,一种鉴定差异显示ESTs简单而有效的方法。在研究基因表达、比较不同环境条件下动物组织的mRNA差异表达等方面具有广阔的应用前景。
The differential expression ESTs in ovarian tissue of little tailed han sheep and tan sheep,were identified with DIG DNA labeling detection technique and reverse northern blotting method. these methods can avoid the pollution of isotope radioactivity,so their security is higher and manipulation is simple.All these showed that reverse northern blotting can quickly and accurately detect differential expression mRNA profiles of different tissues,and can be widely used to study mRNA expression,differential expression of different animal tissues under variant environments.
出处
《生物技术通报》
CAS
CSCD
2005年第2期44-46,共3页
Biotechnology Bulletin
基金
国家自然科学基金资助项目(30270949)
山东省自然科学基金资助项目(Y97D03062)
