摘要
【目的】在大鼠切口痛模型中研究鞘内注射(it)氯胺酮或(和)吗啡对脊髓一氧化氮合酶(NOS)活性和一氧化氮(NO)含量的影响。【方法】雄性SD大鼠64只,随机分为8组, 每组8只, 分别为假手术组, 对照组, 术后氯胺酮治疗组, 术前氯胺酮治疗组, 术后吗啡治疗组, 术前吗啡治疗组, 术后氯胺酮加吗啡治疗组和术前氯胺酮加吗啡治疗组。按Brennan法制成大鼠切口痛模型, 以vonFrey细丝法(机械性痛觉过敏)、热辐射法(热痛觉过敏)和累积疼痛评分法观察疼痛的行为学变化,以分光光度法测定脊髓NOS活性和NO含量。【结果】与假手术组比较,在术后2h时对照组大鼠的vonFrey纤毛刺激缩爪阈值明显降低(P<0. 01),热刺激缩爪潜伏期明显缩短(P<0. 01),累积疼痛评分明显升高(P<0. 01 );与对照组比较,术后/术前吗啡治疗组和术后/术前吗啡加氯胺酮治疗组大鼠的vonFrey纤毛刺激缩爪阈值均明显增加(P<0. 01),热刺激缩爪潜伏期均明显延长(P<0. 01),累积疼痛评分均明显降低(P<0. 01);与假手术组比较, 对照组大鼠脊髓NOS活性和NO含量明显增加(P<0. 01);与对照组比较,术前氯胺酮治疗组、术前吗啡治疗组和术前氯胺酮加吗啡治疗组能使脊髓的NOS活性明显降低, NO含量明显减少(P<0. 01或P<0. 05)。【结论】在大鼠切口痛模型中。
To observe the effects of intrathecal ketamine or(and) morphine on nitric oxide synthase(NOS) activity and nitric oxide (NO) content of the spinal cord in a rat model of incisional pain.Sixty-four male SD rats were divided randomly into eight groups: Sham group, control group, post-incisional/ pre-incisional ketamine group, post-incisional/ pre-incisional morphine group, post-incisional/ pre-incisional ketamine plus morphine group. A PE-10 catheter was inserted into the lumbar subarachnoid space and a 1 cm long incision was made in the plantar area of the left hindpaw ,according to the method of Brennan. Pain behavior was assessed by withdrawal threshold to von Frey filament stimulation intensity, response latency of the hindpaw to radiant thermal and a cumulative pain score 2 h after incision. NOS activity, NO content were measured by spectrophotometry. Withdrawal threshold to von Frey filament stimulation intensity and response latency of the hindpaw to radiant thermal in control group were significantly lower than those in Sham group( P <0.01). Cumulative pain score in control group was significantly higher than that in Sham group( P <0.01). In post-incisional/ pre-incisional morphine group, post-incisional/ pre-incisional ketamine plus morphine group , withdrawal threshold to von Frey filament stimulation intensity and response latency of the hindpaw to radiant thermal were significantly higher than those in control group( P < 0.01 ), cumulative pain score was significantly lower than that in control group ( P <0.01). NOS activity and NO content of the spinal cord increased significantly in control group as compared with those in sham group( P <0.01). NOS activity and NO centent of the spinal cord in pre-incisional ketamine group, pre-incisional morphine group and pre-incisional ketamine plus morphine group were significantly lower than those in the control group( P <0.05 or P <0.01). [Conclusion]In a rat model of incisional pain, antinociceptive effect of preemptive intrathecal morphine or ketamine plus
出处
《医学临床研究》
CAS
2005年第4期433-436,共4页
Journal of Clinical Research
