摘要
比较褐藻胶裂解酶产生菌Alteromonassp .在摇瓶和发酵罐培养过程中生物量、褐藻胶寡糖含量以及褐藻胶裂解酶活性的变化 ,根据其变化确立了通过微生物发酵 膜分离技术结合制备褐藻胶寡糖的条件 ,并对寡糖进行凝胶过滤色谱和薄层色谱分析。用组成为每升含酵母粉 5g、蛋白胨 10g、FeSO4 0 1g、褐藻酸钠 12g、NaCl 1 5g ,pH为7 5的培养基 ,在 2 8℃培养褐藻胶裂解酶产生菌 ,结果表明 ,发酵罐培养 30h ,发酵液寡糖含量达到最大。发酵液通过超滤 纳滤两级膜分离 ,得到褐藻胶寡糖 ,寡糖的回收率和脱盐率分别为 94 0 %和 93 3%。通过凝胶柱分离和TLC分析 ,得到 5个褐藻胶寡糖组分。
Changes in biomass,oligosaccharides content and alginate lyase activity in broth of Alteromonas sp. incubated in shaking flask and fermentation jar were studied. According to those changes, parameters were established for preparing oligosaccharides from alginate by fermentation combined with nano-filtration membrane separation method. Resulting oligosaccharides were analyzed by gel permeation chromatography and thin layer chromatography. One liter culture medium(pH7.5)contains 5g yeast extract,10g peptone, 0.1g FeSO 4,12g sodium alginate and 1.5g NaCl. When incubating at 28℃,the result showed that the optimal fermentation time was 30 h to obtain the highest production of oligosaccharides in the broth. After ultra-filtration and nano-filtration, 94.0 of the total oligosaccharides was recovered from the broth, and meanwhile 93.3 of the salt was removed. Gel permeation chromatography and TLC analyses indicated that the resulting oligosaccharides were composed of five fractions with different degree of polymerization.
出处
《微生物学报》
CAS
CSCD
北大核心
2005年第2期309-311,共3页
Acta Microbiologica Sinica
基金
宁波大学博士基金资助项目 (2 0 0 3 496)
宁波科技局博士基金资助项目 (2 0 0 4A610 0 14 )~~
