摘要
目的通过检测角膜肌成纤维细胞(MFB)特征性标志α-平滑肌肌动蛋白(α-SMA)的表达,探讨PRK和LASIK后早期角膜细胞的增生情况以及对角膜透明度的影响。方法新西兰白兔24只,随机分为两组,每组12只:A组,右眼行PRK手术,左眼为正常对照;B组,右眼行LASIK手术,左眼为正常对照。分别于术后3、10、20、35d取兔眼角膜,部分行光镜和电镜观察;另一部分用于免疫组织化学研究,检测成纤维细胞骨架的特征性标志α-SMA的表达,并计数成纤维细胞活化数量。结果PRK组术眼上皮细胞增生明显,成纤维细胞数量增多,基质浅层胶原纤维排列紊乱,上皮下α-SMA表达明显,表达量与上皮增生数量有关。LASIK组术眼仅角膜瓣周边区域上皮增生,基质层胶原纤维排列无明显紊乱,少量活化的成纤维细胞,角膜瓣边缘可见α-SMA表达。两组中的非手术眼均未见α-SMA表达。结论PRK去除了角膜上皮及前弹力层,术后上皮及浅基质层细胞增生明显,成纤维细胞活化增殖,新生胶原排列不规则,是导致角膜透明度下降的原因。LASIK术后角膜结构的完整性保持较好,除角膜瓣边缘外,其余部位无明显上皮增生,成纤维细胞活化数量少,手术区界面组织增生轻微,术后角膜保持透明。
Objective To assess the proliferation of cells and α-smooth muscle actin (α-SMA ) effect on corneal transparency after photorefractive keratectomy(PRK) and laser in situ keratomileusis ( LASIK). Methods Twenty-four adult New Zealand white rabbits were averagely assigned into group A and group B. PRK was performed on the right eyes of rabbits in the group A and LASIK for group B. All the left eyes were used as control. Eyeball was enucleated and prepared for light and electron microscopy examination in 3,10,20 and 35 days after surgery, and a-smooth muscle actin and the amount of active fibroblasts were assessed using immunohistochemical staining. Results In the PRK group, corneal epithelium cells were proliferated. The numbers of fibroblasts were clearly increased and the collagen fibrils were irregularly arranged, α-smooth muscle actin was expressed in keratocytes repopulated in the central anterior cornea. In the LASIK group, epithelium cells proliferation and α-smooth muscle actin were not seen but corneal flap margin, and the collagen fibrils were organized. The control eyes appeared to be normal. Conclusion After PRK, corneal epithelium cells and Bowman's layer were removed and fibroblasts were actived to produce new collagen. Most anterior stroma showed irregular lamellar structures with an increased number of fibroblasts which induce haze. After LASIK,the corneal structure maintained regular arrangement and had a lighter wound healing response in flap and stromal interface,therefore keeping clear cornea.
出处
《眼科研究》
CSCD
北大核心
2005年第2期121-124,共4页
Chinese Ophthalmic Research
基金
国家自然科学基金资助(30070801)
