摘要
目的:探讨MAGE—A1,-A4基因在大肠肿瘤中的表达及临床意义. 方法:采用免疫组化S-P法,检测正常人大肠黏膜38 例,大肠腺瘤31例,大肠腺癌组织83例中MAGE- A1,-A4蛋白的表达和定位特点. 结果:MAGE—A1蛋白定位于胞质,MAGE—A4蛋白定位于胞质及胞核;二者在正常大肠黏膜组织中几乎均不表达,在大肠肿瘤(大肠腺瘤、大肠腺癌)中均有较高水平的表达;但在大肠腺瘤与大肠腺癌中的表达差异均无显著性;二者在大肠腺瘤中的表达与患者的性别、年龄、组织学类型、是否复发及癌变之间差异均无显著性;二者在大肠腺癌中的表达均与患者的性别、年龄、组织学分级、侵袭深度、有无肝转移之间无统计学意义,均与有无淋巴结转移之间有统计学意义(P<0.01),且均呈正相关关系(PA1<0.01,rsA1=0.287;PA4<0.01,rsA4=0.312). 在有淋巴结转移的大肠腺癌中,MAGE—A1与A4蛋白表达呈正相关关系(P<0.05,rs=0.247). 结论:MAGE-A1,-A4基因可能在大肠肿瘤的发生、发展(尤其是淋巴结转移)中起作用,有望成为诊断大肠肿瘤及其发生淋巴结转移的分子生物学标记物之一.
AIM: To explore the expression and clinical significance of MAGE-A1, MAGE-A4 genes in human colorectal neoplasm. METHODS: MAGE-A1, MAGE-A4 were detected by streptavidin-peroxidase (S-P) immunohistochemistry in 38 normal colorectal mucosal tissues, 31 colorectal adenoma tissues and 83 colorectal adenocarcinoma tissues. RESULTS: MAGE-A1 was located in cytoplasm, whereas MAGE-A4 was observed in both cytoplasm and nucleus. While hardly detected in normal colorectal mucosal tissues, their expression was significantly increased in colorectal neoplasm tissues (P<0.01). However, there was no difference between colorectal adenoma and colorectal adenocarcinoma. The expression of both genes had no relationship with sex, age, histological type, recurrence and malignant transformation of colorectal adenoma, or histological grade, depth of wall invasion and liver metastasis of colorectal adenocarcinomas. However, the expres- sion of both genes was significantly correlated with regional lymph node metastasis in colorectal adenocarcinoma (PA1<0.01, rsA1 = 0.287, PA4<0.01, rsA4 = 0.312). There was correlation between the expression of MAGE-A1 and MAGE-A4 in colorectal adenocarcinoma with regional lymph node metastasis (P<0.05, re = 0.247). CONCLUSION: MAGE-A1 and -A4 genes may play important roles in the occurrence of colorectal neoplasm and the progression of colorectal adenocarcinoma, especially the lymph nodes metastasis. So they could be regarded as two molecular markers for early diagnosis of colorectal neoplasm as well as prediction of regional lymph node metastasis of colorectal adenocarcinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第5期657-661,共5页
World Chinese Journal of Digestology
