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含人甲胎蛋白mRNA部分序列的耐核糖核酸酶病毒样颗粒的构建和表达 被引量:5

Construction and expression of RNase-resisting virus-like par-tides containing partial sequence of alpha-fetoprotein messenger RNA
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摘要 原发性肝癌为一种严重威胁人们健康的恶性肿瘤.甲胎蛋白(AFP)mRNA在监测原发性肝癌的肝外转移以及术后复发方面是一个很好的标志物,也可用于肝癌治疗效果的评价.通常AFP mRNA的检测方法为逆转录巢式聚合酶链反应(RT-PCR)法进行定性检测,或利用凝胶电泳扫描的方法进行半定量检测.近来随着实时荧光PCR技术的发展使得准确定量检测AFP mRNA成为可能.
出处 《中华肝脏病杂志》 CAS CSCD 北大核心 2005年第4期304-306,共3页 Chinese Journal of Hepatology
基金 国家自然科学基金(30371365)首都医学发展 科研基金(2002-3041)
关键词 甲胎蛋白 MRNA 耐核糖核酸酶 病毒样颗粒 构建 表达 细菌噬菌体 原发性肝癌 Alpha-globulins Virion Bacteriophages Cloning vector
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