摘要
目的 为食蟹猴实施人工授精和适时配种提供一种有效的测定排卵期的方法。方法 选择 4～ 6岁性成熟的雌性食蟹猴 30只 ,对其阴道涂片进行动态观察。结果与结论 食蟹猴阴道涂片中主要有角化上皮细胞、中层基底细胞、白细胞和细胞碎片等。分析结果说明 ,白细胞数各时期差异均具显著性 ;角化上皮细胞在月经前期和月经期没有差异 ,但其他各期差异具显著性 ;月经期和月经后期中层基底细胞数目变化差异不显著 ,但其他各期差异具显著性 ;月经前期和月经期的细胞碎片数目相似 ,但其他各期差异具显著性。各时期细胞成份变化规律是 ,角化上皮细胞从月经期到排卵期呈逐渐增加至最高 ,然后逐渐减少 ;而白细胞、中层基底细胞和细胞碎片则恰好相反。
Objective To afford an effective method to determine the ovulatory phase in female Macac a fascicularis for its artificial insemination and hybridization. Meth od 30 female Macaca fascicularis at the age of 4-6 years were selec ted to assess the changes of various types of cells in vaginal smears. R esult and Conclusion The major cell types in the Macaca fascicularis vaginal smears included leucocytes, keratinized epithelial cells, intermediat e and basal cells and cell debris. The amount of leucocytes is significantly dif ferent in various phases of the menstrual cycle(p<0.05). The number of kerat inized epithelial cells is the same in both preovulatory and menstrual phases, but is significantly(p< 0.05) different in other phases of the menstrual cycle. There is no significant dif ference in the number of intermediate and basal cells at menstrual and postovula tory phases, but is significantly(p<0.05) different in other phases of the m en strual cycle. The amount of cell debris is similsr to that of keratinized epithe lial cells. The changes of cells profile in various phases were that the number of keratinized epithelial cells increased gradually to tiptop from menstrual pha se to ovulatory phase and then reduced gradually, but the changes of leucocytes, intermediate and basal cells and cell debris are contrary to that of keratinize d epithelial cells.
出处
《中国实验动物学报》
CAS
CSCD
2005年第1期33-37,i001,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
广西大学动物繁殖研究所与广西区营林开发中心科研合作项目 (编号 :2 0 0 2 -0 5 -2 0 )
广西科学基金项目 (编号 :桂科青 :0 3 3 90 0 5 )
广西大学谭锦球青年科研基金资助项目 (编号 :0 2 2 0 48)。
