摘要
目的探讨γ射线对犬胆管壁增殖性平滑肌细胞Fas基因表达的影响以及与细胞凋亡的关系及意义。方法分别将103钯(103Pd)放射性支架和普通支架植入两组犬的肝外胆管内,术后30d取胆管标本,应用免疫组化法检测犬胆管壁增殖性平滑肌细胞的凋亡情况及其中Fas基因的表达情况,并用计算机图像检测系统检测胆管腔面积。结果放射性支架组犬胆管组织中Fas基因表达水平较普通支架组高。Fas基因高表达的犬胆管增殖性平滑肌细胞凋亡明显,且肝外胆管无明显狭窄。Fas基因低表达的犬胆管增殖性平滑肌细胞无明显凋亡,且肝外胆管有明显狭窄。结论Fas基因表达水平与细胞凋亡的发生和细胞对辐射的敏感性有关,103Pd放射性支架通过增强Fas基因表达,促进犬胆管增殖性平滑肌细胞凋亡,从而抑制犬肝外胆管狭窄。
Objective To study γ-radiation-induced expression of Fas gene and its significance in the apoptosis of proliferative muscle cells of bile duct wall in dog model. Methods The ~ ~103 Palladium (Pd) radioactive stent or general stent was inserted into dog extrahepatic bile duct, and bile duct specimens were ~obtained after 30 days. Expression of Fas and apoptosis of bile duct muscle cells in the control and γ-radiation-~induced groups were determined by immunohistochemical technique. The square of bile duct cavities of two groups were measured by computer ~imaging detecting system. Results The ~expre- ~ssion of Fas was much higher in the bile duct wall of dogs with ~ ~103 Pd radioactive stent than that in dogs with the general stent, and apoptotic muscle cells were more common in Fas highly expreessed than that in the Fas lowly ~experssed subgroup, and no stricture of extrahepatic bile duct was seen in former subgroup. No obvious ~apoptotic muscle cells were observed in the Fas lowly expressed subgroup, however, the dog in this ~subgroup had the obvious stricture of extrahepatic bile duct. Conclusions The level of Fas gene expression was associated with the rate of cell apoptosis in dog bile duct wall, and the ~ ~103 Pd radioactive stent may increase the expression of Fas gene, enhance the apoptosis of proliferative muscle cells of bile duct, and therefore prevent the stricture of extrahepatic bile duct.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2005年第3期150-152,共3页
Chinese Journal of Digestion
