摘要
研究了用β环糊精(βCD)作为流动相手性添加剂,在反相C18板上分离D,L苯丙氨酸对映体的薄层色谱法。流动相最佳组成配比为VβCD饱和溶液∶V甲酸∶V甲醇=9∶2∶1,最佳分离温度为50℃,D,L苯丙氨酸对映体相对比移值α为1.15。
SEPARATION OF ENANTIOMER OF PHENYLALANINE BY REVERSED-PHASE THINLAYER CHROMATOGRAPHY$$$$ FANG Yanhong, WANG Qiong, XU Jinrui (College of Material Science and Engineering, Huaqiao University, Quanzhou 362011, China)Abstract: Racemes of phenylalanine can be separated on RPC18 aluminium sheets by thinlayer chromatography βcyclodextrinformic acidmethanol as developing system in which βcyclodextrin was used as chiral additive. The optimal composition of the mobile phase was βcyclodextrin solution∶formic acid∶methanol=9∶2∶1(V/V/V). The optimal separation temperature was 50 ℃. The values of relative Rf of Dphenylalanine and Lphenylalanine was 1.15. Keywords: Reversedphase thinlayer chromatography; βCyclodextrin; Phenylalanine; Enantiomer
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2005年第4期241-242,244,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
关键词
反相薄层色谱法
Β-环糊精
苯丙氨酸
对映体
Reversed phase thin layer chromatography
β Cyclodextrin
Phenylalanine
Enantiomer
