摘要
为了减小现有的表面等离激元共振传感器的系统漂移和溶液体效应引起的误差,利用四元光电探测器,发展了一种差分SPR传感器,通过对信号的差分运算消除这些误差.测试结果表明, 该仪器有 2个突出的优点:具有 10-5 (°)或 10-8 RIU的高角向分辨率;能同时消除系统漂移和溶液体介电常数效应引起的误差,10min内漂移小于 7×10-5 (°).利用这种传感器,测量浓度为 0 55nmol/L的抗生物素, 得到很好的响应特性,证明该装置可用于生物分子的检测.
In order to reduce the errors caused by systematic drifts and solution bulky effect, a differential surface plasmon resonance (SPR) sensor is developed by using a qudrancell photodetector. Thus the errors can be eliminated through the differential operation on signals. Testing result reveals that the sensor possesses two outstanding advantages; angular resolution is as high as 10-5 degree or 10-8 RIU; simultaneous reductions in systematic drift and refractive effects caused by the change in refractive constant of bulk solution can be achieved. The thermal drift is effectively reduced to less than 7 × 10-5 degree in 10 minutes. With this SPR sensor, avidin of concentration of 0.5 nmol/L was examined and a nice response characteristic was obtained. The result indicates that this device could be widely employed for measuring biomolecules.
出处
《东南大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2005年第2期202-205,共4页
Journal of Southeast University：Natural Science Edition
基金
教育部留学回国人员基金资助项目 (6807031029)
南京航空航天大学基金资助项目(S0472 062)
关键词
表面等离激元共振
抗生物素蛋白
传感器
Error compensation
Laser beams
Optical systems
Photodetectors
Sensors
Thermal effects
