摘要
用2,4-二硝基氯苯保护谷胱甘肽的巯基制出半抗原,再通过戊二醛共价反应使其与牛血清白蛋白表面的氨基结合,经超胶AcA54凝胶层析纯化,制备出有较强免疫原性的谷胱甘肽全抗原。用元素分析、红外光谱及核磁共振表征了半抗原的结构。电泳分析得全抗原分子量平均为87000道尔顿。光谱分析及圆二色谱研究表明其有较强的可见、紫外及荧光特征吸收,且抗原结构的紧密性增强。
The sulfhydryl groups of the reduced glutathione(GSH) have been reacted with2,4-dinitrochlorobenzene to give S-protective glutathione as a hapten, The haptens werecrosslinked with the amino groups on the surface of BSA(Bovine Serum Albumin ) glutaraldehyde,The covalent conjugates are GSH specific antigens of high immunogenicity.The antigens were found to be homogenious and have a molecular weight of 87000 dalton asdetermined by gel-electrophoresis method.Spectral and circular dichroism measurementsshowed that the antigens possess special vis-UV spectra , fluorescence nature with aincreased compact of antigen structure.
出处
《应用化学》
CSCD
北大核心
1994年第5期87-90,共4页
Chinese Journal of Applied Chemistry
关键词
谷胱甘肽
过氧化物酶
催化抗体
glutathione peroxidase
catalytic antibody
specific antigen
preparation