摘要
目的: 探讨人膀胱肿瘤细胞BIU -87培养上清对树突状细胞(DC)的生成和功能的影响。方法: 分离培养外周血单个核细胞(PBMC)来源的DC, 实验组加入BIU -87细胞的培养上清液, 以正常培养的DC作为对照组, 应用流式细胞术检测在实验组和对照组不同条件下培养的DC上表面标志的表达, 并用改良的MTT比色法检测和对比两组所培养的DC激活同种异体T细胞的能力及其差别。结果: DC与BIU- 87细胞的上清液共培养后, CD1a、CD83及CD86均呈低表达。实验组与对照组相比较, DC的成熟受到抑制, 对同种异体T细胞激活的作用明显降低 (P<0. 05 )。结论: BIU- 87细胞的上清液可抑制DC的生成及相关表面标志的表达, 这可能是导致膀胱肿瘤患者DC数量减少和功能降低的原因。
AIM: To explore the effects of culture supernatant of bladder tumor cell lines BIU-87 on the development and function of peripheral blood mononuclear cell (PBMC)-derived dendritic cells. METHODS: The culture supernatant of BIU-87 cells was collected and used as the conditional culture medium for PBMC-derived DCs culture in experiment group. Normal cultured DCs were used as control group. The phenotypes of DCs in experiment group and in control group were analyzed by FACS. The capacity and difference to stimulate allogenetic T cells of PBMC-derived DCs from the two groups were evaluated by MTT colourimetry. RESULTS: Phenotypic analysis showed that DCs co-cultured with culture supernatant of BIU-87 cells expressed lower levels of CD1a, CD83 and CD86. Moreover, as compared with control group, the maturation of DCs was inhibited, and the capacity to stimulate allgentic T cell proliferation by DCs descended(P<0.05). CONCLUSION: The culture supernatant of BIU-87 cells can inhibit the development and function of DCs. It is possible to result in a reason of decreased the number of DCs and its functional descent in the patients with bladder tumor.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2005年第2期237-239,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(No. 30271300)
