摘要
[目的 ]对急性驰缓性麻痹病例粪便标本分离的脊髓灰质炎病毒进行型内鉴定。[方法 ]采用PCR RFLP技术 ,对分离毒株进行鉴定。[结果 ] 1999— 2 0 0 2年福建省脊灰分离株在 3D区有 5株Ⅱ型病毒发生重组或变异 ,表现为Ⅲ型 ;在VP1区有 3株发生变异 ,不同于经典Ⅱ型病毒 ,未发现脊灰野病毒。[结论 ]PCR RFLP技术能快速确认脊灰毒株的基因特征 ,初步区分脊灰病毒是否为野病毒或发生重组变异 。
To identify poliovirus isolated from feces of acute flaccid paralysis case type in gene. Isolated strains were identified by PCR-RFLP method. Recombination or variation (some fragments) occurred in 5 Type -Ⅱ poliovirus strains in 3D region and they displayed Type -Ⅲ in Fujina (1999-2001). Variation occurred in 3 strains in VP1 region and they were different from Sabin Ⅱ and no wild poliovirus found. Gene characteristics of poliovirus can be identified quickly by PCR-RFLP method. The wild poliovirus or the recombination and variation can be preliminarily distinguished and the method is useful for polio prevention and control.
出处
《海峡预防医学杂志》
CAS
2004年第6期11-13,共3页
Strait Journal of Preventive Medicine
基金
福建省卫生厅青年科研基金资助 (项目号2 0 0 2 1 19)
