摘要
目的 :了解输血传播病毒 (TTV)在孕产妇血清、乳汁及新生儿脐血中的感染情况 ,探讨其在母婴间的传播途径及生后 1年期间的带菌情况。方法 :应用套式 PCR对 40 0例正常孕产妇分娩前的血清及其新生儿的脐血血清标本、产妇的乳汁配对进行 TTV DNA检测 ,对阳性的母亲及其新生儿进行 6个月、 12个月随访 ,对 TTV DNA成对阳性的母血、母乳及其婴儿的血进行克隆和序列测定 ,比较分析母婴 TTV感染株之间核苷酸的序列同源性。结果 :62例孕妇血清、 4例新生儿脐血清、 2 0例母乳检测出 TTV DNA,孕母 TTV感染率 15.5% (62 / 4 0 0 ) ,出生时母婴 TTV传播率 6.5% (4/ 62 ) ,阳性母亲乳汁 TTV DNA检出率 3 2 .2 % (2 0 / 62 ) ,经 6个月、 12个月随访孕母血 TTV DNA阳性的 42对母乳喂养的母婴 ,母血清 TTV DNA阳性率分别为2 1.3 % (9/ 4 2 )、 82 .1% (2 3 / 2 8) ,婴儿血清 TTV DNA阳性率分别为 40 .5% (17/ 4 2 )、 2 8.5% (8/ 2 8)。配对比较母婴间 TTV感染株序列同源性为 97.4%～ 99.8%。结论 :TTV可经非血液途径传播 ,TTV感染可自然转阴 。
Objective:By investigating the prevalence o f TTV infection in mother's sera, milk and infant's cord blood to describe the m other-to-child transmission route of TTV and the bacteria carrying situation i n one year after baby born Methods:TTV DNA was detected by nested PCR in sera ,cord b lood and breast milk samples from 400 mother-infant pairs TTV DNA positive mo thers and infants were followed up for 6 months and 12 months TTV DNA PCR prod uct samplified from mother blood, milk and newborn blood of positive mother-inf ant pairs were cloned and sequenced Compared the DNA sequence between mother a nd infant TTV infection Results:By means of PCR, TTV DNA was detected in 62 of mothers' sera samples, 4 of 400 cord blood samples and 20 of 400 milk samples,respectively So the mother TTV infection rate is 15 5%(62/400), the mother- to-infant TTV transmitted rate is 6 5%(4/62) and the detective rate of TTV DNA in positive mother's milk is 32 2%(20/62) 42 mother-infant pairs of positiv e mother's sera and breast fed infant were followed up for 6 and 12 months The positive rate of mother'ssera is 21 3%(9/42) and 82 1%(23/28) respectively in 6 and 12 months The pos itive rate of infant's sera is 40 5%(17/42) and 28 5%(8/28) respectively The TTV nucleotide homology between mother and infant is 97 4% to 99 8% Conclusion:TTV can be transmitted by non-blood way such a s breast feeding TTV infectors can change to normal naturally or become a heal thy bacteria carrier
出处
《中国妇幼保健》
CAS
2004年第2期83-85,共3页
Maternal and Child Health Care of China
关键词
输血传播病毒(TTV)
母婴传播
随访研究
Transfusion Transmitted Virus (TTV)
Mother-to-child transmis sion
Follow-up study
