摘要
目的 检测邢台和北京 2城市健康献血员血清中TT病毒 (TTV)感染情况 ,并结合北京市慢性乙型肝炎和慢性丙型肝炎病人TTV感染情况进行比较 ,以阐明TTV在健康人群中的感染情况及其存在的意义。方法 用改良非编码区 (UTR)PCR和N2 2PCR法 ,分别检测健康献血员、慢性乙型肝炎和慢性丙型肝炎病人血清中TTVDNA。结果 UTRPCR检测表明 ,健康献血员TTVDNA检出率为 98 3% ,而 6 0例慢性乙型肝炎和 80例慢性丙型肝炎患者血清中TTVDNA检出率均为 10 0 % ;N2 2PCR在上述人群中TTVDNA检出率分别为 32 6 % ,35 0 %和4 2 5 %。结论 TT病毒在人群中有很高的感染率 ,但N2 2PCR检测的结果与UTRPCR相比明显偏低 ,二者间有显著性差异。在健康人群和慢性肝炎病人中 。
Objective TTV DNA was detected in serum samples obtained from volunteer blood donors living in Xingtai and Beijing City,from patients wit h chronic hepatitis B as well as chronic hepatitis C living in Beijing City,in a n attempt to shed light on the prevalence of TTV infection and the role of TTV infection.Methods Modified UTR PCR and N22 PCR were performed,respe ctively,to test TTV DNA in serum samples from 227 blood donors,60 chronic hepati tis B patients and 80 chronic hepatitis C patients.Results TTV DNA was identified by UTR PCR in 98.3% of 22 7 volunteer blood donors,in 100% both of 60 chronic hepatitis B patients and 80 chronic hepatitis C patients.On the other hand,among blood donors,chronic hepati ts B patients and chronic hepatitis C patients,TTV DNA was detected by N22 PCR a t a frequency of 32.6% (73/227),35.0%(21/60),42.5%(34/80),respectively.Conclusion The prevalence of TTV DNA detected by UTR PCR was extremely high in examined population,the results obtained by N22 PCR was mu ch less frequently in the same population.The significant difference existed in the prevalence of TTV DNA detected by the two different PCR systems.However,the prevalence of TTV infection detected by same PCR system was similar between hea lthy blood donors and hepatitis patients.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第10期1230-1231,共2页
Chinese Journal of Public Health
基金
留学回国人员科研启动基金 (教外司留 [2 0 0 2 ] 2 4 7号 )
