摘要
为满足转目的基因的需求,探索一种能够将外源基因转入银杏细胞,并得以稳定表达和遗传的转基因方法以及选择合适的载体.用基因枪将带有和CaM35S启动子与NOS终止子相连接的GUS基因的质粒pBI221打入银杏细胞,以X-Gluc为底物检测GUS的活性,所得结论为:GUS基因被转入了银杏细胞并在银杏细胞中得到了表达,但该基因没有稳定存在于银杏细胞中.
Ginkgo biloba is a tall tree species with luxuriant leaves and huge biomass. If a purpose gene could be transferred into this tree species and if the transferred gene could express and be heritable steadily in callus cells of Ginkgo biloba,the production of plant species with the transferred purpose gene would be high. To meet the demands of gene transfer of an objective gene, a suitable vector should be screened to carry the extraneous objective gene into the callus cell of Ginkgo biloba. Plasmid pBI221 carrying GUS gene linked with the CaM35S promoter and the NOS terminator was shot into callus cells of Ginkgo biloba by the particle gun bombardments, and the activity of GUS gene was examined by X-Gluc substrate. It was showed by the results that GUS gene was successfully transferred into callus cells of Ginkgo biloba, and the transferred GUS gene was expressed in the callus cells of Ginkgo Biloba, however, the transferred gene did not stably exist in the callus cells of Ginkgo biloba.
出处
《西南林学院学报》
2004年第4期1-3,共3页
Journal of Southwest Forestry College
基金
中国科学院1999国际合作项目资助.
