摘要
花生根瘤菌类菌体含有吸Ｈ２酶，以Ｏ２或亚甲蓝为电子受体均表现吸Ｈ２活性。类菌体还原减氧化的吸收差示光谱在４２４、５００、５２０、５５０、５６０、５８５及５９５ｎｍ处呈现吸收峰，表明细胞色素ｃ、ｂ、和ｏ参与Ｈ２的氧化过程。ＣＮ－明显影响５２０、５５０、５６０ｎｍ处的吸收峰，意味着有些细胞色素ｃ和ｂ对ＣＮ－敏感，在Ｈ２代谢中充当末端氧化酶的作用。抗霉素Ａ、ＨＯＮＯ、ＣＮ－、Ｎ３－和ＤＢＭＩＢ均强烈抑制吸Ｈ２活性，但鱼藤酮不抑制吸Ｈ２活性。说明细胞色素ｂ、ｃ、ａ和泛配参与Ｈ２氧化的电子传递，而ＮＡＤＨ脱氢酶不参与。花生根瘤菌类菌体的Ｈ２氧化系统是个复杂具分支的电子传递体系。
An investigation was conducted to identify electron transport carriersthat participated the oxidation of H2 in bacteroids of Rhizobium arachis．H2-reduced mi-nus O2-oxidized absorption difference spectra in bacteroids showed peaks at 424,500,520,550,560,585 and 595nm,indicating the involvement of c-,b-,a and o-types ofcytochromes,The absorption peaks at 520,550,560nm were diminished a lot in the pres-ence of CN-This showed that some of c-and b-type cytochromes were CN- sensitiveand therefore might play a role as terminal oxidases in H2 metabolism. H2-uptake was in-hibited by antimycin A,HONO,CN-,N3-and DBMIB,not by rotenose,Our resultsindicate that ubiquinone and some types of cytochromes are involved in the oxyhydro-gen reaction which is a complex,branched transport system.
