摘要
目的 研究青石棉纤维作用于A5 4 9细胞后IL 8蛋白和基因表达水平及酪氨酸激酶抑制剂PD980 5 9对IL 8表达的影响。方法 用定量RT PCR法测定青石棉诱导A5 4 9细胞后的IL 8mRNA水平 ;用免疫反应法测定A5 4 9细胞培养上清中IL 8蛋白水平。结果 不同浓度青石棉刺激A5 4 9细胞后 ,培养上清中IL- 8释放量明显增加 ,细胞中A5 4 9IL- 8mRNA表达显著上升 ,使用酪氨酸激酶抑制剂PD980 5 9可明显抑制IL- 8的蛋白及mRNA表达水平。结论 青石棉可诱导IL- 8的高表达 ,而这种表达可为PD980 5 9所抑制 ,提示IL -8的表达源于胞浆中MAPKs信号通路。
Objective\ This study was to investigate the effect of crocidolite fibe rs on IL-8 protein and mRNA levels in A549 cells cultured supernatant and the r o le of tyrosine kinase inhibitor PD98059 on IL-8 expression. Methods Real Time PC R and ELISA were employed to dectect A549 cellular IL-8 mRNA and supernatant IL - 8 protein, respectively. Results After incubating cells with 10 0μg/ml of crocid olite, IL-8 protein releasing in supernatant was significantly increased and IL - 8 mRNA was also higher than that of control, the differences were statistically si gnificant(P<0.05),however both IL-8 protein and mRNA was abrogated by pret reati ng cells with tyrosine kinase inhibator PD98059. Conclusion IL -8 was shown as ov erexpressoin and could be decreased by PD98059. This indicated that MAPK signal pathway is involved in the process IL-8 expression.
出处
《卫生研究》
CAS
CSCD
北大核心
2005年第2期141-143,共3页
Journal of Hygiene Research
基金
美国国家环境保护局
北卡罗来那大学联合资助 (No.CR82 952 2 )
河南省卫生厅创新人才基金项目 (No .2 0 0 4 0 2 )
