摘要
目的:观察哮喘大鼠支气管细胞间黏附分子-1(ICAM鄄1)和核因子-κB(NF鄄κB)的表达、炎性细胞浸润的变化,探讨雷公藤多甙干预后的影响。方法:18只Wistar大鼠分为哮喘组(A组),雷公藤多甙治疗组(T组)和正常对照组(C组),采用免疫组化方法分别观察ICAM鄄1、NF鄄κB在3组大鼠支气管上皮的表达。结果:①哮喘组支气管上皮ICAM鄄1、NF鄄κB的表达显著高于对照组(P<0.05);②治疗组支气管上皮ICAM鄄1、NF鄄κB的蛋白表达与哮喘组相比显著降低(P<0.05);③哮喘大鼠支气管NF鄄κB与ICAM鄄1蛋白表达比较呈显著正相关(r=0.832,P<0.01)。结论:哮喘大鼠模型支气管NF鄄κB对于ICAM鄄1蛋白表达具有重要的调控作用。雷公藤多甙对于哮喘的治疗机制之一可能通过下调受NF鄄κB调控的ICAM鄄1表达,减少肺组织中炎性细胞的浸润而实现。
Objective:To investigate the effects of tripterygium polyglucoside on the ICAM-1,NF-κB and inflammatory cells in bronchioles of asthmatic rat. Methods:Eighteen male Wistar rats were randomly divided into three groups:asthmaic rat(A), asthmaic rat treated with tripterygium polyglucoside(T) and control group(C), The ICAM-1 and NF-κB levels in bronchioles of all groups were measured by immunohistochemistry. Results:① The ICAM-1 and NF-κB in bronchioles of group A were higher than that of group C(P < 0.05);② Compared with asthmatic rats,the ICAM-1 and NF-κB in bronchioles of group T was abated(P < 0.05);③A significant positive correlation existed between the expression of NF-κB and that of ICAM-1 in the bronchioles of asthmatic rats. Conclusion:NF-ΚB plays an important action on the ICAM-1 expression in the bronchioles of asthmatic rats. Tripterygium polyglucoside may alleviate the airway inflammation by down-regulating the expression level of ICAM-1 controlled by NF-κB.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第4期252-254,F002,共4页
Journal of Nanjing Medical University(Natural Sciences)
