摘要
目的 :研究诱导型、内皮型一氧化氮合酶 (iNOS、eNOS)在喉鳞癌中的表达及其与肿瘤血管形成的关系。方法 :应用免疫组化方法检测 4 5例喉鳞癌患者手术切除石蜡包埋标本的iNOS、eNOS、血管内皮生长因子 (VEGF)的表达 ,应用Ⅷ因子相关抗原 (F8)显示血管内皮细胞。根据F8阳性的血管内皮细胞计数来测定肿瘤微血管密度 (MVD)。以 11例喉乳头状瘤、5例正常喉黏膜作为对照。结果 :①iNOS和eNOS在喉鳞癌中的阳性表达率分别为 5 7. 8%、6 6 . 7% ,而喉乳头状瘤呈低表达 ,正常喉黏膜无表达 ;②两型NOS的表达强度与喉鳞癌病理分级有显著相关性 (P <0 . 0 1) ,即iNOS和eNOS的表达随着喉鳞癌恶性程度的加深而增强 ;③在eNOS不同表达强度之间MVD的差异有极显著性 (P <0. 0 1)即随eNOS表达强度增加MVD值也增加 ,而在iNOS不同表达强度之间MVD没有显著差异 (P >0. 0 5 ) ;④VEGF在喉鳞癌中的阳性表达率为 4 8. 9% ,显著高于喉良性肿瘤 ,正常喉黏膜不表达 ;eNOS表达与VEGF表达之间有极显著相关性 (P <0 . 0 1) ,iNOS表达与VEGF表达之间无相关性 (P >0 . 0 5 )。结论 :NO在喉鳞癌的生长分化和血管生成中起到促进作用 ,eNOS的催化作用在肿瘤血管形成过程中占优势。
Objective:To study the expression of inducible NOS (iNOS) and endothelial NOS (eNOS) in laryngeal squamous cell carcinoma (LC) and their relationship with angiogenesis.Methods:Tissue sections from 45 specimens of laryngeal squamous cell carcinoma (LC) were examined immunohistochemically for protein expression of iNOS,eNOS,and vascular endothelial growth factor (VEGF),with 11 cases of laryngeal papilloma (LP) and 5 nomal laryngeal mucosa as contrast group.Microvessal density (MVD) was counted by endothelial cells immunostained by Facyor Ⅷ-related Antigen (F8).Results:①Positive iNOS and eNOS immunostaining was detected in 57.8% and 66.7% of LC respectively,with low immunostaining in LP and negetive immunostaining in nomal laryngeal mucosa.②There was a correlation between the expression of NOS and the differentiation in LC.③The expression of eNOS was in direct proportion to microvessal density.④The positive rate of VEGF was 48.9% in LC,higer than LP,and negetive in nomal laryngeal mucosa.There was a correlation between the expression of eNOS and VEGF in LC,and no relationship between iNOS and VEGF.Conclusions:NO plays a important role in growth,differentiation,and angiogenesis of laryngeal squamous cell carcinoma,and the catalytic reaction of eNOS has a predominant role during angiogenesis.
出处
《内蒙古医学杂志》
2005年第2期97-101,F004,共6页
Inner Mongolia Medical Journal
关键词
喉肿瘤
一氧化氮合酶
血管形成
Laryngeal neoplasms
Nitric oxide synthase
Angiogenesis
