摘要
目的:研究新发现的核激素受体辅调节因子(coregulator) NRIF3 的受体特异性的分子机理。方法:酵母双杂合体转化分析,选择典型菌株作β 半乳糖苷酶定量分析。结果:我们的结果提示一个双区结合模型,即单个NRIF3分子同时利用C端的LXXIL(受体结合功能区,即 RID1)和 N端的 LXXLL(RID2)与 TR或 RXR作用,RID1和RID2之间的距离对受体和NRIF3相互作用的亲和力有重要影响。结论:辅调节因子 NRIF3 受体特异性与其结构有关。
Objective: To study molecule mechanism of distinct receptor specificity of a novel coregulator for nuclear hormone receptors, which designates NRIF3. Methods: Mutagenesis analysis, the yeast two-hybrid assay, and quantitative β-galactosidase assays of selected typical colonies were used. Results: The results suggested a bivalent interaction model, where a single NRIF3 molecule utilizes both the C-terminal LXXIL (receptor-interacting domain 1 \) and the N-terminal LXXLL (RID2) modules to cooperatively interact with TR or RXR (presumably a receptor dimer), with the space between RID1 and RID2 playing an important role in influencing the affinity of the interactions. Conclusion: Receptor specificity of coregulator NRIF3 is relevant to its domain structure.
出处
《武汉大学学报（医学版）》
CAS
2005年第2期194-198,共5页
Medical Journal of Wuhan University
关键词
辅调节因子
受体特异性
分子机理
Coregulator
Distinct Receptor Specificity
Molecule Mechanism
