摘要
目的 研究结直肠癌患者外周血中CRD BPmRNA的表达,探讨其作为结直肠癌标志物的可行性。方法 用套式荧光实时PCR对 64例结直肠癌患者、20例胃肠道良性疾病患者和 25例健康成人进行外周血CRD -BPmRNA的检测,并对阳性PCR产物进行克隆、测序。结果 结直癌患者中, 28(43 8% )例检测到CRD BPmRNA表达,在良性病和健康人均无表达,两组差异显著(χ2 =26. 493,P<0. 001)。CRD -BPmRNA的表达与结直肠癌的Dukes分期有显著相关性(χ2 =8. 195,P<0. 05)。结论 在结直肠癌患者的外周血中,能够检测到CRD- BPmRNA的表达,CRD- BPmRNA可作为外周血中结直肠癌细胞的肿瘤标志物用于临床诊断。
Objective To investigate the expression level of CRD-BP (coding region instability determinant-binding protein) mRNA in peripheral blood of colorectal cancer patients and estimate the availability of CRD-BP as a marker of colorectal cancer.Methods Peripheral blood samples were collected from 64 colorectal cancer patients,20 non-cancerous patients and 25 healthy adults.The expression level of CRD-BP mRNA in peripheral blood was detected by fluorescent real-time nested reverse transcriptase-polymerase chain reaction (RT-PCR).PCR product was cloned into PUC19 vector and sequenced.Results In research group 28 samples (43.8%) showed positive expression of CRD-BP mRNA while none was positive in controls with an obviousdifference (χ2=26.493, P <0.001).There was a significant correlation between CRD-BP mRNA expression and Dukes stage of colorectal cancer(χ2=8.195, P <0.05).Conclusion CRD-BP mRNA may be detectable in peripheral blood of colorectal cancer patients and should be a useful tumor marker in peripheral blood for clinical diagnosis of colorectal cancer.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2005年第2期120-122,共3页
Chinese Journal of Clinical Laboratory Science
