摘要
目的 探讨单核细胞趋化蛋白 - 1 (MCP 1 )在慢性阻塞性肺疾病 (COPD)发病机制中的作用及糖皮质激素的干预作用。方法 2 4只Wistar大鼠随机分为 3组 :正常对照组、COPD模型组和泼尼松干预组。采用单纯熏香烟法建立COPD模型 ,干预组隔日泼尼松灌胃。支气管肺泡灌洗计数支气管肺泡灌洗液 (BALF)中细胞总数与肺泡巨噬细胞 (AM)数 ,酶联免疫吸附法 (ELISA)测定BALF和血清中的MCP 1及肿瘤坏死因子- α(TNF- α)浓度。肺组织切片观察形态学改变 ,采用图像分析系统测定肺平均内衬间隔 (MLI)、平均肺泡数 (MAN)和肺泡腔面积与总面积比 (PAA)。结果COPD模型组MLI、PAA较正常对照组增高 ,而MAN低于对照组 (P <0 . 0 1 )。COPD模型组BALF中MCP 1、TNF α浓度、细胞总数和AM计数均高于正常对照组 (P <0 . 0 1 )。血清中MCP 1的升高与正常对照组相比无统计学差异 (P >0 . 0 5 )。泼尼松干预组MLI和PAA较COPD模型组降低 ,而MAN高于COPD模型组 (P <0. 0 1 ) ,BALF中MCP 1及TNF α浓度 ,细胞总数及AM计数与COPD模型组比较均有下降 (P <0 . 0 1 )。BALF中MCP 1浓度与AM数目和TNF α浓度 (r =0 . 86 1 ,P <0 . 0 1 ;r =0 . 86 8,P <0 . 0 1 ) ,以及TNF- α浓度与AM数目 (r=0 . 84 ,P <0 . 0 1 )均呈密切正相关 。
Objective To explore the role of monocyte chemoattractant protein-1 (MCP-1) in the pathogenesis of chronic obstructive pulmonary disease (COPD) and the effects of glucocorticoid.Methods Wistar rats were randomly allocated into three groups:normal control,COPD group,and prednisone preventive group.Rat COPD model was established by exposing the animals to cigarette smoke daily.Prednisone was given to rats in prednisone preventive group through stomachal injection on alternative days.After four months bronchoalveolar lavage (BAL) was performed.Total cell counts and alveaoler macrophage (AM) counts in BALF were examined and the levels of MCP-1 and TNF-α in BALF and serum were measured by ELISA.Lung tissue sections stained by HE were observed to study morphological alternation in terms of MLI,MAN and PAA.Results MLI and PAA in COPD group were higher than those in control group while MAN was just on the contrary.Except the level of MCP-1 in serum (P>0.05),the levels of MCP-1 and TNF-α,total cell counts and AM counts in BALF of COPD group were significantly higher than those in control group (P<0.01).After the treatment with prednisone MLI and PAA were significantly decreased while MAN was increased.Significant decrease in the levels of MCP-1 and TNF-α,total cell counts and AM counts in BALF were found in prednisone preventive group as compared to those in COPD group (P<0.01).Positive correlations were demonstrated among the level of MCP-1,the level of TNF-α and AM counts in BALF in COPD group,but not between the levels of MCP-1,TNF-α in serum and AM counts.Conclusions A close correlations between MCP-1 in BALF and airway inflammation in COPD was found which implys an important role of this chemottractant factor in the pathogenesis of COPD.Prednisone may relieve airway inflammation in COPD by inhibiting MCP-1,TNF-α and AM.
出处
《中国呼吸与危重监护杂志》
CAS
2005年第2期126-129,i001,共5页
Chinese Journal of Respiratory and Critical Care Medicine
