摘要
RNA沉默是一种在真核生物体内普遍保守的、通过核酸序列特异性的相互作用来抑制基因表达的调控机制. RNA沉默的一种重要生物学效应是防御病毒的侵染,而针对寄主的这种防御机制,许多植物病毒已演化通过编码RNA沉默的抑制子来克服这种防御反应. 目前,已从植物、动物和人类病毒中鉴定了20多种RNA沉默的抑制子,围绕抑制子的鉴定和作用机理研究已成为病毒学研究的一个热点. 对RNA沉默抑制子的发现、鉴定方法、作用机理及与病毒病症状形成的关系、动物病毒的沉默抑制子等方面的最新进展做了综述,并对沉默抑制子的应用和存在的问题进行了讨论.
Conserved in many eukaryotic cells, RNA silencing is a mechanism that suppresses gene expression through RNA-mediated sequence-specific interactions. In plants' RNA silencing plays an important role against virus infection. A counter-defensive strategy in plant viruses has evolved by encoding suppressor proteins to overcome RNA silencing. Up to date, over twenty suppressors encoded by plant, animal and human viruses had been identified. A hot topic in virology has been focused on the identification of suppressors of RNA silencing and the mechanism for inducing silencing. The discovery and identification of viral suppressors of RNA silencing, possible mechanisms involved in RNA silencing suppression and its relationship with symptom formation of viral diseases, and suppressors of animal viruses were reviewed. The applications of these suppressors in plant biological and biotechnological research were also discussed.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2005年第3期210-216,共7页
Progress In Biochemistry and Biophysics
基金
国家杰出青年基金(30125032)
国家自然科学基金(30270062)资助项目~~
