摘要
目的 探讨急性期川崎病 (KD)血清 s IL - 2 R、CRP、IL - 6、IL - 8、TNF-α的变化。方法 应用终点散射比浊法、双抗体夹心酶联免疫吸附法 (EL ISA)检测 30例急性期 KD患儿口服阿斯匹林和静脉注射丙种球蛋白前后血清 s IL-2 R、CRP、IL- 6、IL- 8、TNF- α的含量。结果 治疗前 s IL- 2 R、CRP、IL- 6水平高于对照组 (P<0 .0 1 ,P<0 .0 1 ,P<0 .0 5 ) ,治疗后 s IL- 2 R、CRP、IL- 6含量下降 ,与治疗前相比差异有显著性 (P<0 .0 5 ) ;IL- 8、TNF- α在治疗前后与对照组相比差异无显著性 (P>0 .0 5 )。结论 s IL- 2 R、CRP、IL- 6参与了 KD的疾病过程 ,阿斯匹林和大剂量静脉注射丙种球蛋白影响KD患者血清 s IL- 2 R、CRP、IL- 6水平 ,为治疗方案提供了依据。
Objective To explore the changes of soluble interleukin-2 receptor(sIL-2R), C-reactive protein(CRP), interleukin-6(IL-6), interleukin-8(IL-8) and tumor necrosis factor-α(TNF-α) in acute phase of Kawasaki disease(KD) in children. Methods CRP was measured by scatter turbidimetry, and cytokines level were measured by enzyme-linked immunosorbent assay (ELISA).Results The pretreatment serum levels of sIL-2R, CRP and IL-6 were significantly higher in patients with KD than healthy controls (P<0.01,P<0.01,P<0.05); while the posttreatment serum levels were significantly lower than the pretreatment levels(P<0.05).There was no significant difference in IL-8 and TNF-α between KD patients and healthy controls (P>0.05). Conclusion sIL-2R,CRP and IL-6 are involved in the pathogenesis of KD. Aspirin and intravenous gamma globulin therapy will reduce the serum levels of sIL-2R, CRP and IL-6 in KD children. The results accordingly buttress their use in these patients.
出处
《西部医学》
2005年第2期171-172,共2页
Medical Journal of West China
