摘要
用葡萄球菌Ａ蛋白（ＳＰＡ）和链球菌Ｇ蛋白（ＳＰＧ）Ｓｅｐｈａｒａｓｅ亲和层析分离提纯衣原体脂多糖（ＬＰＳ）和合成衣原体属特异性多糖抗原单克隆抗体ＩｇＧ，方法简便快速，分离后的ＩｇＧ纯度高，优于ＬＰＳ特异性抗原Ｓｅｐｈａｒｏａｅ亲和层析法。
Monoclonal IgG antibodies against chlamydial
LPS and artificial chlamydial genus-specificpolysaccharide were purified by protein A-and Protein
G-Sepharose,which was easier and quickerthan specific antigen LPS-Sepharose affinity
chromatography.The purity of IgG in the eluate washigh.
关键词
亲和层析
葡萄球菌
蛋白
链球菌
affinity chromatography
protein
A-and protein G-sepharose
IgG
