摘要
用非放射性标记的ＨＢＶ－ＤＮＡ探针检测３７１份至少有一项ＨＢＶ免疫学标志阳性血清和５１０份对照血清的ＨＢＶ－ＤＮＡ。结果表明：５１０份对照血清和７９份仅抗－ＨＢｓ阳性血清的ＨＢＶ－ＤＮＡ均为阴性，８２份ＨＢｓＡｇ阳性、ＨＢｅＡｇ阳性血清的ＨＢＶ－ＤＮＡ检出率为１００％，１０３份ＨＢｓＡｇ阳性、ＨＢｅＡｇ阴性血清的检出率为７９．６％，７６份ＨＢｓＡｇ阳性。ＨＢｅＡｇ阴性、但抗－ＨＢｅ阳性血清的检出率为８０．３％，２９２份ＨＢＶ不同感染阶段血清总检出率为７５％。非放射性分子杂交法可直接检测出ＨＢＶ的复制，由于该法亦可定量，故还可用于判断血清传染程度和血清病毒的含量。
method which
marked by non radioactive digoxigenin HBV-DNA was used to check up theserum of 371 cases
in which at least orle item of HBV was positive,and 51 0 of normal control serum。The
HBV-DNA of 510 normal control and 79 patients serum which only has anti-HBs positive,were
negative, The positive rate of the test of the serum of 82 cases(HBsAg+,HBeAg+)was 100%。
The rate of 103 cases (HRsAg+,HBeAg-)was 79.6%。 The rate of 76 cases was 80.3%, in
whichHBsAg positive, HBeAg negative, but the anti-HBe positive,The total rate of HBV of 292
cases was75%in different infectious stages. The method of nonradioactive molecular
hybridization can check out the duplicate of HBV direct-ly, It can be rationed and it also can be
used to judge the extent about the serum infection and the con-tent of the serum virus.This
method is quite simple and easy and might be used in basic unit。
关键词
DNA
探针
乙型肝炎病毒
分子杂交
nonradioactive
digoxigenin HBV-DNA
HBV
molecular hybridization
