摘要
目的 :构建一系列新的用于解脂耶氏酵母的杂合启动子 方法 :采用PCR技术将pXPR2的一段上游激活序列 (UAS - 1B)进行了离体串联重复 ,得到了一系列拷贝数不同的串联重复体 ,并将其克隆到了pTEF启动子的上游 结果 :通过酶切鉴定 ,确证得到了含有UAS - 1B重复单元数目不等的杂合启动子 结论 :本方法实用可行 ,并构建了一系列杂合启动子 。
Objective: To construct a series of hybrid promoters. Methods: Adopting PCR techniques to construct tandem repeats of the UAS-1B regions of pXPR2 promoter and incorporate them into upstream of the minimal promoter encompassing the TATA box and ATG region of the promoter pTEF. Results: restriction enzyme digestion identified hybrid promoters encompassing different repeats of UAS-1B were constructed successfully. Conclusion: The method is feasible and the construction of these hybrid promoters laid a good foundation for the construction of high expression lever vectors of yeast Yarrowia lipolytica.
出处
《宜春学院学报》
2004年第6期4-6,18,共4页
Journal of Yichun University
基金
国家"8 63"计划资助项目 (2 0 0 2AA2 2 70 11)
