摘要
目的 提高EGF- PE35KDEL融合蛋白的表达量。方法 设计合成含优化密码子的EGF序列 ,定向克隆于pET2- 8a- EGF PE35KDEL的NcoⅠ和NdeⅠ位点 ,构建表达质粒pET2 8a pBEGF PE35KDEL。将两种表达质粒转化至BL2 1中 ,经IPTG诱导 ,比较表达量。结果 优化密码子的EGF- PE35KDEL表达量要高于未优化的EGF -PE35KDEL。结论 优化密码子能促进EGF -PE35KDEL的表达。
Objective To enhance the expression of EGF-PE35KDEL fusion protein.Methods Design and synthesize an EGF sequence containing the optimized codon and directi onally cloned to the Nco Ⅰ and Nde Ⅰ sites of plasmid pET28a-EGF-PE35KDEL to construct an expression plasmid pET28a-EGF-PE35KDEL.Plasmids pET28a-EGF-PE3 5KDEL and pET28a-EGF-PE35KDEL were transformed into E.coli BL21 for expres sion under induction of IPTG,and their expression levels were compared.Results The expression level of EGF-PE35KDEL containing the optimized codon was signifi cantly higher than that containing no the optimized codon.Conclusion The optimization of codon promoted the expression of EGF-PE35KDEL fusion protei n. [
出处
《中国生物制品学杂志》
CAS
CSCD
2005年第2期107-108,110,共3页
Chinese Journal of Biologicals
