摘要
目的探讨胸腺树突状细胞及相关细胞因子在重症肌无力(MG)发病中的作用。方法采用免疫荧光双标法对11例胸腺瘤伴MG患者、6例单纯胸腺瘤、5例正常胸腺组织中S100(+)、HLADR(+)的树突状细胞进行检测,同时用组织匀浆、ELISA法检测胸腺组织中IL2、IL12含量。结果(1)树突状细胞主要分布在胸腺皮质、皮髓交界处,在胸腺瘤伴MG患者胸腺髓质也有一定分布。(2)S100(+)、HLADR(+)树突状细胞数量在胸腺瘤伴MG患者与单纯胸腺瘤患者间、单纯胸腺瘤患者与正常胸腺之间差异有显著性(P<005)。(3)IL2、IL12含量在胸腺瘤伴MG患者与正常胸腺之间差异有显著性(P<005);IL12含量在胸腺瘤伴MG患者与单纯胸腺瘤患者间亦有显著性差异(P<005)。结论胸腺树突状细胞以多种形式参与MG发病。
Objective To investigate the changes of distribution and quantity on S-100(+) HLA-DR(+) dendritic cells and their related cytokine in thymus in patients with myasthenia gravis. Methods (1) The patients with both myasthenia gravis and thymoma were 11 cases, only with thymoma was 6 cases and normal thymus of adult were 5 cases. Immunohistochemical staining for S-100 and HLA-DR were performed according to the specimens. Dendritic cells positive on both S-100 and HLA-DR were counted. (2)The quantity of IL-2 and IL-12 in thymus were evaluated with ELISA. Results (1) Dendritic cells positive on both S-100 and HLA-DR located mainly at cortical areas of the thymus. However, there were some positive cells in the medulla in myasthenia gravis patients.(2) Positive cells per high power (10×40) were respectively 7.78, 0.92, 0.96 in group of thymoma with myasthenia gravis, thymoma without myasthenia gravis, and normal thymus. (3) The quantity of IL-12 and IL-2 in thymus of patients with both myasthenia gravis and thymoma was higher than that of patients with normal thymus (P<0.05). The quantity of IL-12 in thymus of patients with both myasthenia gravis and thymoma was also higher than that of patients with thymoma without myasthenia gravis (P<0.05). Conclusions The quantitive of dendritic cells and their related cytokine may play an important role in development of myasthenia gravis through different ways.
出处
《中国神经免疫学和神经病学杂志》
CAS
2005年第2期70-72,F005,共4页
Chinese Journal of Neuroimmunology and Neurology
基金
国家自然科学基金资助项目(30270485)
