摘要
本文采用了无机盐与有机溶剂相结合的方法制备纤维素酶。TichodermakoningiP2固体培养基的抽提液中，加入4％氯化钙，就能达到硫酸按法的同样效果，除去酶液中的一些杂质。进一步沉淀酸时，与直接用有机溶剂沉淀比较，溶剂用量减少了30％．溶剂采用异丙醇较佳，粗酶制剂的产率以羧甲基纤维素酶活（CMCA）表示接近100％，使滤纸崩溃的能力与原酶液相似。
A method has been devised, using CaCl2 and organic solvent as precipitam, for the preparation of crude cellulase from the culture of Trichod erma koninqi P2. When precipitating impurity, the amount of 4% CaCl2 was added. Another advantage of this method was that the amount of isopropyl alcohoi used decreased 30per cent in the presence of CaCl2. The yield rate of CMCA activity was closed to 100% and the activity of filter paper in crude cellulase was similar to that of the culture filtrate.
出处
《纤维素科学与技术》
CAS
CSCD
1994年第2期59-63,共5页
Journal of Cellulose Science and Technology
关键词
纤维素酶
沉淀
制备
酶制剂
cellulase, Precipitation, preparation
