摘要
目的建立顽痹乐丸的质量控制标准。方法采用TLC法对补骨脂、葛根、白芍、炙甘草进行定性鉴别;采用薄层扫描法对补骨脂中异补骨脂素进行含量测定,层析板:高效硅胶G板,展开剂:正己烷-醋酸乙酯(8∶2),采用反射法单波长锯齿扫描,光束狭缝:0.4×0.4mm,线性参数SX=3,扫描波长300nm。结果鉴别项下阴性对照无干扰,专属性强;异补骨脂素在0.064～1.536μg间呈良好的线性关系,r=0.9980,平均回收率为100.7%,RSD为2.0%。结论该方法可用作顽痹乐丸的质量控制方法。
Objective To set up the quality standard of Wanbile Wan. Method s Fructus Psoraleae, Radix Puerariae, Radix paeoniae Alba and Radix Glycyrrhiza e Preparatae in Wanbile Wan were identified by TLC .Isopsoralen was determined b y TLC scanning. The chromatographic conditions were: high performance silica gel G plate as lamellated plate, n- hexane - ethyl acetate (8 ∶ 2)being the exp ander, serrated scanning with single- wavelength reflection, beam stricture of 0.4× 0.4 mm, linear parameter SX=3 and scanning wavelength at 300 nm. Results The TLC spots were highly specific, clear and concentrated without interferen ce of the presence of negative controls. Isopsoralen showed a good linearity wit hin the range of 0.064～ 1.536 μ g, r=0.9980, average recovery rate was 100.7 % , and RSD was 2.0% .Conclusion This method can be used for the quality co ntrol of Wanbile Wan.
出处
《中药新药与临床药理》
CAS
CSCD
2005年第2期118-121,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
关键词
顽痹乐丸
补骨脂
葛根
白芍
炙甘草
异补骨脂素
薄层色谱法
Wanbile Wan
Frutus Psoraleae
Radix Puerariae
Radix paeoniae A lba
Radix Glycyrrhizae Preparate
Isopsoralen
Thin layer chromatography
