摘要
黄连属（Ｃｏｐｔｉｓ）植物根茎中小檗碱的组织化学研究以往都采用新鲜材料切片。试验应用碘化汞钾处理黄连（ＣｏｐｔｉｌｓｃｈｉｎｅｎｓｉｓＦｒａｎｃｈ．）根茎，使其中小檗碱沉淀后，石蜡法制片。观察结果表明，黄连根茎中小檗碱主要分布在皮层、髓和髓射线薄壁组织细胞内，木化细胞的细胞壁中也有小檗碱。根茎的基部小檗碱含量少，中部和分枝内较多；节部比节间含量多。在不同年生根茎中，二年生苗根茎的一些细胞内小檗碱开始积累，以后逐年增加，至第六年达小檗碱积累高峰，以后根茎内小檗碱含量不再增加。根据小檗碱在根茎中的分布和积累规律，对黄连栽培和采收提出一些建议。
For determing the distribution and concentration of berberine in Coptis rhizomes,sectionswere cut from the fresh rhizomes and placed immediately in the various reagents in the previousresearches.But in present research,the fresh rhizomes were cut into 0.2-0.3cm long and treated with Hg-KI to crystallize berberine in tissues before the sections,which could be protected for a long time,were cut by rotating microtome,and three results were obtained.(1)Abundant crystallization of berberine was occurred in the parenchyma of the cortex,the pith and the pithrays. The berberine was also detected in walls of the xylem constituents,the stone cells and thebast fibers,(2)A smaller number of berberine crystals were observed in the section of basal partof a main rhizome than in those of middle part and its branches ,and the amount of berberine innodal part is more than that in cylindrical part.(3)The sections of various age rhizomes showedthat berberine began to concentrate in cells of rhizome of 2-year-old plants,and then it increasedactually in each year until its amount was at top level in 6-year-old plants. According to previousconclusions,we had offered some valuable suggestions on cultivation and harvest of Coptis chinensis Franch.
出处
《西北植物学报》
CAS
CSCD
北大核心
1994年第3期164-168,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金
关键词
黄连
根
茎
小檗碱
组织化学
Coptis chinensis Franch.,rhizome,berberine,histochemistry
