摘要
采用不连续的聚丙烯酰胺凝胶电泳法，结合组织特异性染色和酶谱区带活性扫描技术，对家鸡的心、肝、肾、脾、胸肌、睾丸、卵巢、脑、眼晶体共９种组织器官的酯酶同工进行了测定，发现不同组织器官酯酶同工酶均有分布，呈现广谱性，但谱带模式又各不相同，具有很明显的组织器官特异性。酯酶谱带活性与各组织器官所执行的功能相吻合，说明酯酶在调节组织器官代谢中发挥重要作用。
The discontinuous polyacrylamide gel electrophoresis(PAGE )with the tissue specifcity satining and zymogram band activity scanning techniques was used to detect the esterase isozymes of heart,liver,kideny,spleen,breaest,testis,ovary,brain and eyes of cbicken. The results show that the esterase isozyme was in all the tissuse and organs studied and had the wide ranging distribution. However,the zymogram pattern of every tissue and organ was different,i.e. it had the obvious tissue and/or organ specificity. The activity of the esterase bands had a close correspon- dence with the function which the tissue and/or orgon had caried out,which demonstated that the esterases played an important role in the regulation of the metabolisms of tissues and organs
出处
《西北农业学报》
CSCD
1994年第2期36-40,共5页
Acta Agriculturae Boreali-occidentalis Sinica
关键词
鸡
家鸡
酯酶
组织器官
同功酶
Chicken
Esterase isozme
PAGE
Tissue and organ specificity
