摘要
采用分光光度法和淀粉凝胶电泳法初步研究了鲮鱼冷休克前后脑乙酰胆碱酯酶(AchE)活力和三种酶的同工酶类的动态变化。并根据鲮鱼在冷休克期间(7—6℃)脑AchE活力显著降低和肝脏组织乳酸脱氢酶(LDH)同工酶活性明显升高以及苹果酸脱氢酶(MDH)、酯酶(EST)同工酶类出现酶活性变化的情况,讨论了导致鲮鱼耐寒能力差的某些生化因素。同时提出脑AchE可以作为评定鲮鱼冷休克期间中枢神经系统受害程度的一种生化指标。
The dynamic changes in the activity of brain acetylcholinesterase (AchE) and the isozymes of three enzymes during the period of mud carp's cold shocking were preliminarily investigated through the methods of spectrophotometry and starch gel vertical electrophoresis. According to the remarkable lowering of brain AchE and the notable raising of the activities of lactate dehydrogenase (LDH) isozymes in the liver tissue, as well as the changes of the activities in malate dehydrogenase (MDH) and esterase(EST) isozymes in the tissues of mud carp which were under cold shocking, we discuss some biochemical factors correlated with the genetic trait of low temperature endurance in mud carp and suggest that the limiting activity of brain AchE could be used as a useful biochemical index showing the damage to the central nervous system, which in turn causes cold shocking and dying with metabolic disorders in the mud carp.
