摘要
比较研究了鲫鱼异倍体细胞系(CAB-80)、团头鲂尾鳍细胞系(BCC)、大鳞副泥鳅雌核发育单倍体胚胎细胞系(PHG)、草鱼胚胎细胞系(GCE)、草鱼尾鳍细胞系(GCRF-2)、草鱼肾细胞系(GCK-84)及其四个克隆对草鱼呼肠孤病毒(GCRV)的敏感性。证实了这些细胞(PHG除外)在不同程度上对GCRV敏感,其中以GCK-84的敏感性最强。这表明,在体外培养条件下,GCRV并无严格的种族特异性。用经GCK-84传代的病毒感染草鱼种,能复制出典型的出血病症状。用GCK-84检测了病毒在GCK-84、GCRF-2、CAB-80、BCC和PHG中的滴度(TCID_(50/ml)),其值分别为8.24,7.36,2.90,2.15和1.33。4个克隆与肾细胞系对病毒的敏感程度亦不尽相同,其滴度在6.3到9.32之间变化。上述结果对细胞工程抗病育种预示有较大的潜在意义。在电镜下可见GCRV对被感染的细胞造成了严重的破坏。病毒为平均直径58nm的球形颗粒,具有一个高度电子密度的核心,平均直径约为38nm。病毒在细胞中的分布方式有三种:即散布于细胞质中的、呈晶格状包于一膜状结构中的和整齐或不整齐地聚集在一起但无膜包裹的。
The susceptibility to grass carp reovirus (GCRV) of CAB-80, BCC, PHG, GCE GCTF-2 and GCK-84 (these cell lines were derived from crucian carp blastula embryos, herbivorous bream caudal fins, loach haploid gastrula embryos, grass carp gastrula embryos, grass carp caudal fins and grass carp kidney, respectively), as well as four clones isolated from GCK-84, were analyzed comparatively. Sensitivity of various degrees was confirmed in the infected cell lines (except PHG) among which GCK-84 was the most sensitive. It is demonstrated that GCRV, in vitro, has no strict species specificity. Typical symptom of muscular hemorrhage could be replicated when fingerlings of grass carp were infected with the virus which had been propagated in GCK-84. The virus produced by GCK-84, GCRF-2, CAB-80, BCC and PHG was titred in GCK-84 cells. The titers (TCID50/ml) were 8.24, 7.36, 2.90, 2.15 and 1.33 respectively. Sicells. difference was also observed in the four clones and GCK-84, with the titers ranging fro6.3 to 9.32. The results prescnted above predict the relative potential significance in the disease-resistant breeding of fish cell engineering.Serious damages in GCRV-infected GCK-84 cells could be seen with electron microscopy. The observed virus was in the shape of spherical particles having a size of 58 nm in diameter and a core with high electronic density approximately 38 nm in diameter on the average. The distribution of virions in cells could be divided into three patterns: existing individually in cytoplasm, congregating in large crystalline aggregates bound by membranceous structures, gathering together in an orderly or disorderly arrangement without a surrounding membrane.
